Nrf2抗体
Nrf2抗体-核因子E2相关因子2多克隆抗体 (ab31163)
概述
- 产品名称 Nrf2抗体-核因子E2相关因子2多克隆抗体
- 产品描述
- 测试过的应用 IF,IHC/ICC,WB
- 种属反应性 牛,鸡,人,小鼠,大鼠
- 别名 红系衍生2抗体
HEBP1抗体
NFE2相关因子2抗体
NF2L2_HUMAN抗体
NFE2相关因子2抗体
NFE2L2抗体
Nrf2抗体
NRF2抗体
核因子抗体
属性
- 产品形式 液体
- 保存说明 4°运输，分装后保存在-20℃，避免反复冻融。
- 保存缓冲液 1×PBS, pH 7.4, 0.02%叠氮化钠
- 浓度 0.5-1mg/ml
- 纯度 Protein A 纯化
- Clonality 多克隆
- 研究领域 表观遗传学和核信号,细胞生物学,代谢,心血管
应用
| 应用 | 评级 | 使用说明 |
|---|---|---|
| IHC-P | 5 | 1/100. 在IHC染色前，需要做热抗原修复 |
| ICC/IF | 3 | 根据试验调整稀释比例. |
| IHC-Fr | 根据试验调整稀释比例. PubMed: 17142801 | |
| WB | 4 | 1/1000.检测到的条带在68kDa（预测的分子量为68kDa） |
验证数据
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody (ab31163)ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunocytochemistry/ Immunofluorescence-Nrf2 antibody(ab31163)ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Nrf2 antibody (ab31163)All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
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